The Consurf analysis method was used. It can quantitatively score the conservation of each amino acid residue among all the variants of CepA and helps to identify functional regions as they use the concept that critical functional sites evolve slowly. The result shows MSA along with a conservation score. Consurf also generated structure figures with color according to the scale on the surface of the enzyme. This figure was used to analyze any variable regions surrounding the active or catalytic site that may be responsible for the change in the configuration of the enzyme undergoing substitution. .